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Athmakuri Tharak, Harishankar Kopperi, Manupati Hemalatha, Uday Kiran, C. G. Gokulan, Shivranjani Moharir,  View ORCID ProfileRakesh K Mishra,  View ORCID ProfileS Venkata Mohan


Wastewater-based epidemiology (WBE) of SARS-CoV-2 emerged as an advantageous method to study the infection dynamics at substantial population level. A temporal glimpse at sewage viral genome helps as diagnostic tool to understand the viral spread at community level. In this study, for the long-term epidemiological surveillance, we monitored the SARS-CoV-2 genetic material in domestic sewage by adopting the longitudinal sampling to represent a selected community (∼1.8 lakhs population which occupies 1.79% of the total population of Hyderabad city) to understand the dynamics of infection. Dynamics and spread of COVID-19 outbreak within the selected community were achieved by studying the longitudinal sampling for a specific period of time. WBE also promotes clinical scrutiny along with disease detection and management, in contrast to an advance warning signal to anticipate outbreaks.

Competing Interest Statement

The authors have declared no competing interest.

Funding Statement

The work was supported by Council of Scientific and Industrial Research (CSIR) New Delhi India in the form of project entitled Testing for COVID-19 in wastewater as a community surveillance measure (6/1/COVID-19/2020/IMD)

Author Declarations

I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.


The details of the IRB/oversight body that provided approval or exemption for the research described are given below:

CSIR-IICT, Hyderabad

All necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived.


I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance).


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